巴西橡胶树胶乳和悬浮细胞中MVA和MEP代谢途径基因的表达分析

MVA和MEP代谢途径是植物类异戊二烯代谢途径的两条重要次生代谢途径。该研究利用荧光定量PCR技术,分析了橡胶树胶乳和橡胶树花药愈伤组织来源的悬浮细胞中MVA代谢途径和MEP代谢途径中关键基因的表达水平,同时分析了茉莉酸的结构类似物冠菌素(coronatine,COR)对悬浮细胞中Hb AACT3,Hb HMGR4,Hb HMGR5,Hb DXS2,Hb DXR和Hb SQS1基因表达的调节作用。结果表明:在MVA代谢途径中,基因Hb AACT1,Hb AACT2,Hb HMGS1,Hb HMGS2,Hb HMGR1,Hb HMGR3,Hb MVK,Hb PMK,Hb MVD1,Hb MVD2和IPP下游代谢基因Hb IPPI1和Hb FDPS1在胶乳中的表达量要相对高于其在悬浮细胞中的表达量,然而橡胶树悬浮细胞中MEP代谢途径基因Hb DXS1,Hb DXS2,Hb DXR,Hb CMS1,Hb CMS2,Hb CMK,Hb MCS1,Hb MCS2,Hb HDS,Hb HDR和鲨烯合酶基因Hb SQS1的表达水平要相对高于胶乳。而且COR能不同程度地上调Hb HMGR5,Hb HMGR4,Hb SQS1,Hb DXS2和Hb DXR基因的表达水平。该研究结果为探索利用橡胶树悬浮细胞体系研究次生代谢合成调控以及生产活性次生代谢产物奠定了基础。

Expression analysis of MVA and MEP metabolic pathways genes in latex and suspension cells of Hevea brasiliensis

The MVA and MEP metabolic pathways are two important plant isoprenoid metabolic pathways in plants. The expression of genes respectively in MVA and MEP secondary metabolic pathways were analyzed in the latex and suspension cells from anther-derived callus of Hevea brasiliensis by using qRT-PCR technology. In addition, expression changes of HbAACT3, HbHMGR4, HbHMGR5, HbDXS2, HbDXR and HbSQS1 genes were further analyzed in the suspension cells under COR treatment. The results demonstrated that expressions of HbAACT1,HbAACT2,HbHMGS1,HbHMGS2,HbHMGR1,HbHMGR3,HbMVK,HbPMK,HbMVD1,HbMVD2 in MVA metabolic pathway and HbIPPI1 and HbFDPS1 genes involved in IPP utilization were relatively higher in latex than that in suspension cells, while HbDXS1,HbDXS2,HbDXR,HbCMS1,HbCMS2,HbCMK,HbMCS1,HbMCS2,HbHDS and HbHDR in MEP metabolic pathway and HbSQS1 were relatively higher in suspension cells than that in latex. Moreover, HbHMGR5, HbHMGR4, HbSQS1, HbDXS2 and HbDXR genes were induced highly or to some degree in suspension cells by COR application. This study lays a foundation for further utilization of suspension cells to analyze secondary metabolism regulation as well as to produce bioactive compounds from anther-derived callus of Hevea brasiliensis in the future.