Development of subprotoplasts from in vitro-grown tobacco pollen tubes |
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Authors: | M. Kroh B. Knuiman |
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Affiliation: | (1) Department of Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands |
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Abstract: | Summary Plasmolyzed pollen tubes of Nicotiana tabacum each released one to three subprotoplasts from their tips when treated with wall-degrading enzymes. Wall regeneration and the further development of the subprotoplasts were studied by both light and electron microscopy. Karyoplasts and cytoplasts incubated in a poor culture medium regenerated a cell wall within 60 min; cellulose microfibrils and callose were shown to be present. In 30%–40% of the cells, one-third of which were nucleate, cell chains and tubes developed by polar growth in a ratio of about 11. They sometimes reached a length 7–9 times the diameter of the former subprotoplast within 5 h of incubation. With longer incubation periods the cell wall became two-layered, its ultrastructure resembling that of the pollen tubes. The capacity of cytoplasts to regenerate a wall and develop cell chains and tubes can be explained by the properties ascribed to the cytoplasm of pollen tubes.Extended version of part of a contribution (poster) presented at the 14th International Botanical Congress, Berlin (West), July 1987. |
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Keywords: | Cell chain formation Cell wall regeneration Cytoplast Karyoplast Nicotiana (pollen tube subprotoplast) Tube formation |
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