Mitochondrial H+-ATPase: Identification of Subunits of the F0 Subcomplex that Contact Membrane Lipids |
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| Authors: | Zaitseva L G Ovchinnikova T V Vodovozova E L Molotkovsky Yul G Polyakov N B Titov M I Esipov S E Grinkevich V A |
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| Institution: | (1) Russian Academy of Sciences, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, ul. Miklukho-Maklaya 10/16, GSP Moscow, 117997, Russia;(2) Chair of Bioorganic Chemistry, Biological Faculty, Moscow State University, Vorob'evy gory, Moscow, 119899, Russia |
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| Abstract: | The subunits of the F0 membrane sector of bovine heart mitochondrial H+-ATPase that contact the lipids of the mitochondrial inner membrane were identified with the use of specially synthesized proteoliposomes that contained active mitochondrial H+-ATPase and a photoreactive lipid, which was 1-acyl-2-12-di-azocyclopentadiene-2-carbonylamino)-12-14C]dodecanoyl]-sn-glycero-3-phosphocholine, 1-acyl-2-11-( 125I]diazoiodocyclopentadiene-2-carbonyloxy)undecanoyl]-sn-glycero-3-phosphocholine, or 1-acyl-2-12-(diazocyclopentadiene-2-carbonylamino)dodecanoyl]-sn-glycero-3-phosphocholine, where acyl is a mixture of the residues of palmitic (70%) and stearic (30%) acids. An analysis of the cross-linked products obtained upon the UV-irradiation of these proteoliposomes indicated that subunits c and a of the F0 membrane sector contact the lipids. The crosslinked products were identified by SDS-PAGE and MALDI mass spectrometry. |
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| Keywords: | mitochondrial H+-ATPase subunits of the F0 membrane sector photoreactive lipids MALDI mass spectrometry of membrane proteins |
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