Cooperative Non-enzymatic Base Recognition and the Stability of the G-U Wobble Pair |
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Authors: | S. K. PODDER |
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Affiliation: | 1.Max-Planck-Institut für Physikalische Chemie,G?ttingen;2.Molecular Biology Group, Department of Biochemistry,Indian Institute of Science,Bangalore,India |
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Abstract: | INVESTIGATIONS of paired complementary polynucleotides containing occasional mismatched bases have indicated that these bases are looped out of the double helical regions thereby making the helix unstable. But in a system containing mismatched G and U bases, no such definite conclusion could be drawn1–4—stoichiometry and Tm values of such complexes can be interpreted in terms of the formation of a G-U wobble pair4. Recently the complexes formed by self-complementary block oligomers interrupted by mismatched bases, for example, AnXUn (X=G, C and U), have been studied in detail5·6. It seems that stacking interactions play a much more dominant role in the stabilization of double helices than was previously thought. Thus the extent of looping out and of non-Watson-Crick base pairing can hardly be assessed independently. |
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