Production of potent antidiabetic compounds from shrimp head powder via Paenibacillus conversion |
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Institution: | 1. Institute of Research and Development, Duy Tan University, Da Nang, 550000, Viet Nam;2. Department of Chemistry, Tamkang University, New Taipei City, 25137, Taiwan;3. Life Science Development Center, Tamkang University, New Taipei City, 25137, Taiwan;1. Key Laboratory for Green Chemical Technology of Ministry of Education, R&D Center for Petrochemical Technology, Tianjin University, Tianjin 300072, China;2. Collaborative Innovation Center of Chemical Science and Engineering, Tianjin 300072, China;1. College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China;2. School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, China;3. School of Medicine, Xiamen University, Xiamen, Fujian, 361102, China;1. School of Lifescience, Taizhou University, 1139 Shifu Rd., Taizhou 318000, China;2. Taizhou Z-starpharm Co.Ltd., Taizhou 318000, China |
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Abstract: | Natural α-glucosidase inhibitors (aGIs) are of great interest as an efficacious and safe therapy for type 2 diabetes, which is an ongoing global health issue. The aim of this study is to utilize shrimp head powder (SHP), an abundant and low-cost material, for the biosynthesis, isolation, and identification of active antidiabetic compounds. SHP was efficiently converted to aGIs via Paenibacillus sp. TKU042 fermentation. Fermented SHP (fSHP) by this strain possesses high pH stability, and stronger yeast α-glucosidase inhibitory activity (92%) than that of acarbose (60%). aGI productivity increased more than 2-fold after optimization (from 225 U/mL to 560 U/mL). Further bioactivity-guided isolation of two major active compounds were identified as nicotinic acid and adenine. Notably, these inhibitors were non-sugar-based moiety aGIs, which is newly isolated and identified from fSHP in this study. In the tests of specific enzyme inhibitory activity, adenine showed highly specific inhibition against yeast α-glucosidase (IC50 = 22 μg/mL); nicotinic acid demonstrated good effect on rat α-glucosidase (IC50 = 70 μg/mL); while acarbose possessed efficient effect on bacteria, rice, and rat α-glucosidases (IC50 = 0.03–108 μg/mL). The current results suggest that it is cost-effective to produce potent aGIs from SHP via Paenibacillus conversion, and these active constituents may be useful in type 2 diabetes management. |
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Keywords: | Shrimp heads Marine processing Antidiabetes Nicotinic acid Adenine |
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