Autoradiographic demonstration of gastrin binding sites in rat gastric mucosa |
| |
| Authors: | M Nakamura M Oda K Kaneko Y Yonei N Tsukada H Komatsu M Tsugu M Tsuchiya |
| |
| Institution: | 1. Department of Diagnostic Pathology, National Cancer Center Hospital, Tokyo, 104-0045, Japan;2. Department of Gynecology, Niigata Cancer Center Hospital, Kawagishicho, Niigata, 951-8566, Japan;3. Department of Basic Pathology, National Defense Medical College, Tokorozawa, Saitama, 359-8513, Japan;4. Department of Pathology and Laboratory Medicine, Omori Red Cross Hospital, Tokyo, 143-8527, Japan;5. Department of Pathology, Shin-Yurigaoka General Hospital, Kawasaki, Kanagawa, 215-0026, Japan;6. Center for Preventive Medicine, Fukuoka Sanno Hospital, Fukuoka, 814-0001, Japan;7. Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, 569-8686, Japan;8. Department of Obstetrics and Gynecology, Osaka Medical and Pharmaceutical University, Takatsuki, Osaka, 569-8686, Japan;9. Department of Obstetrics and Gynecology, Juntendo University Faculty of Medicine, Tokyo, 113-8431, Japan;10. Department of Obstetrics and Gynecology, Faculty of Medicine, The University of Tokyo, Tokyo, 113-8655, Japan;11. Department of Obstetrics and Gynecology, Ehime University, Graduate School of Medicine, Toon, Ehime, 791-0295, Japan;12. Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, 160-0016, Japan;13. Department of Obstetrics and Gynecology, Shinshu University School of Medicine, Matsumoto, 390-8621, Japan;14. Gynecology Service, National Hospital Organization Kyushu Cancer Center, Fukuoka, 811-1395, Japan;15. Department of Obstetrics and Gynecology, Perinatal Medical Center, Kagoshima City Hospital, Kagoshima, 890-8760, Japan;p. Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, 951-8510, Japan;q. Department of Gynecology, Hokkaido University Hospital, Sapporo, Hokkaido, 060-8638, Japan;r. Department of Obstetrics and Gynecology, Faculty of Medicine, Kyushu University, Fukuoka, 812-8582, Japan;s. Department of Obstetrics and Gynecology, The Jikei University School of Medicine, Tokyo, 105-8461, Japan;t. Department of Obstetrics and Gynecology, Graduate School of Medicine, University of the Ryukyus, Uehara Nishihara, Okinawa, 903-0125 207, Japan;u. Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, Sendai, Miyagi, 980-8575, Japan;v. Department of Reproductive Medicine, Chiba University Graduate School of Medicine, Chiba, 260-8670, Japan;1. Department of Pathology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan;2. Department of Pathology, Center Hospital of the National Center for Global Health and Medicine, Tokyo, Japan;3. Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan;1. Department of Gastroenterology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou Province, China;2. Department of Thyroid and Breast Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou Province, China |
| |
| Abstract: | The location of 125I-iodotyrosyl gastrin I binding sites in rat gastric mucosa was studied. Peptide specificity was demonstrated by competitive binding studies through the addition of a large dose of cold human gastrin I or cholecystokinin-octapeptide. Autoradiography of the stomach tissue was carried out by freeze-drying, embedding in Epon, wet-sectioning with ethylene glycol, and dry-mounting the emulsion film by means of the wire-loop method to prevent loss of the labeled substance. Specific binding sites for gastrin were found on parietal and chief cells, whereas few binding sites were seen on the surface mucous or mucous neck cells. Binding sites on the parietal cells were dispersed in the cytoplasm, while those on the chief cells were found near the basal plasma membrane. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
