Biofilm fermentation of iturin A by a recombinant strain of Bacillus subtilis 168 |
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Authors: | Rahman Mohammad Shahedur Ano Takashi Shoda Makoto |
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Institution: | Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan. rahman@res.titech.ac.jp |
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Abstract: | Bacillus subtilis 168 produces thin and fragile biofilm in the static culture, however, it was found out that its transformant B. subtilis RM/iSd16 containing wild sfp, itu operon and degQ, which produced lipopeptide antibiotic iturin A, produced thick and much stable biofilm. Production of iturin A by RM/iSd16 in biofilm was almost two times higher compared to that in the submerged culture at 28 degrees C. |
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