Comparative filter binding study of H5 to nucleosome core particles,H1, H5 depleted chromatosomes and DNA fragments |
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| Authors: | S. Pennings S. Muyldermans L. Wyns |
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| Affiliation: | (1) Laboratory of General Biology, Free University, Paardenstraat 65, B-1640 Sint-Genesius-Rode, Belgium;(2) Present address: Department of Biological Chemistry, School of Medicine, University of California, Davis, 9516 Davis, CA, USA |
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| Abstract: | The filter-binding technique with PEI treated glass fiber is used to study the interaction of histone H5 to core particles, chromatosomes and DNA derived from it. By working at very low concentrations of interacting particles we are able to study the effective binding process independent of interfering insoluble complexes. The interactions are characterized by a very high affinity. An intrinsically higher affinity of H5 for cores and chromatosomes versus chromatosome derived DNA is demonstrated. Both chromatosomes and DNA derived from these bind about twice the amount as compared to core particles, which saturate at about one H5 per core particle.Abbreviations GH5 globular domain of histone H5 - PEI polyethyleneimine |
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| Keywords: | chromatosome histone H5 lattice binding nucleosome core particles |
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