Factors affecting protoplast yield of the carrageenophyte Solieria filiformis (Gigartinales,Rhodophyta) |
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Authors: | Juan Luis Gómez Pinchetti Mats Björk Marianne Pedersén Guillermo García Reina |
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Institution: | (1) Instituto de Algología Aplicada, Universidad de Las Palmas de Gran Canaria, Box 550, 35214 Las Palmas, Islas Canarias, Spain;(2) Department of Physiological Botany, Uppsala University, Villavägen 6, S-752 36 Uppsala, Sweden |
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Abstract: | Summary The effect of age, pH of the culture medium, pre-treatment of tissues, enzymes sources and enzymatic adaptability of phycophages fed with a monospecific diet were analyzed on the protoplast yields of the red seaweed Solieria filiformis (Kützing) Gabrielson. New apices from fast growing plants showed the highest protoplast yields. The protoplast yield decreased when the pH of the culture medium increased from 6.0 to 9.0. Crude extracts from the abalone Haliotis coccinea canariensis Nordsieck, fed with Solieria filiformis thalli for three months in combination with cellulysin, released the highest number of viable cells and protoplasts. Yields ranged from 1.0 to 8.5 x 106 protoplasts per gram of fresh weight.Abbreviations AAP
abalone acetone powder
- Bis-Tris
Bis(2hydroxyethyl)imino-tris(hydroxymethyl)methane
- CMC
carboxymethyl cellulose
- EDTA
ethylenediaminetetraacetic acid
- EGTA
ethylene glycol-bis(ß-aminoethyl ether) NNN N -tetraacetic acid
- FDA
fluorescein diacetate
- FW
fresh weight
- Hepes
N-2-hydroxyethylpiperazine-N -2-ethanesulfonic acid
- Tris
Tris(hydroxymethyl)aminomethane |
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Keywords: | Protoplast Solieria filiformis Enzymes Haliotis coccinea canariensis Culture |
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