Mapping of quantitative trait loci associated with protein expression variation in barley grains |
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Authors: | Katja?Witzel Christof?Pietsch Marc?Strickert Andrea?Matros Marion?S?R?der Winfriede?Weschke Ulrich?Wobus Email author" target="_blank">Hans-Peter?MockEmail author |
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Institution: | 1.Leibniz Institute of Plant Genetics and Crop Plant Research,Gatersleben,Germany;2.Institute of Plant Nutrition and Soil Science,Christian-Albrechts-University,Kiel,Germany;3.KWS LOCHOW GMBH,Einbeck,Germany;4.University of Siegen,Siegen,Germany |
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Abstract: | Barley (Hordeum vulgare) is an important cereal crop grown for both the feed and malting industries. Hence, there is great interest to gain deeper
insight into the determinants of grain nutritional quality in order to improve the assessment of new traits. Two-dimensional
gel electrophoresis was employed for the characterization of the grain proteome of doubled-haploid introgression lines (IL)
representing a wild barley genome (Hordeum spontaneum Hs213) within a modern cultivar background (H. vulgare cv. Brenda). Proteome maps were subjected to differential cluster analysis and revealed ILs with similar or different protein
expression patterns compared to the Brenda parent. A total of 51 quantitative trait loci for protein expression (pQTL) were
detected, and proteins underlying these pQTL were further examined by mass spectrometry. Identification was successful for
49 of the segregating spots and functional annotation of proteins revealed that most proteins are involved in metabolism and
disease/defence-related processes. Among those, multigene families of glyceraldehyde-3-phosphate dehydrogenases, heat shock
proteins, peroxidases, and serpins were identified. Overall, eight pQTL signals were discovered in two independently grown
sets of plants. The mapped spots included protein disulfide isomerase, α-amylase inhibitor BDAI, NADP malic enzyme, adenosine
kinase and peroxidase BP1. Specific marker information of proteins involved in developmental events and protein storage as
well as in disease- and defence-related processes now allows for targeted breeding approaches to improve the grain quality
in barley. |
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