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Coupling of histone mRNA levels to radioresistant DNA synthesis in ataxia-telangiectasia cells |
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| Authors: | Dr Martin F. Lavin Jane Houldsworth Sharad Kumar Janet L. Stein Gary S. Stein |
| Affiliation: | (1) Department of Biochemistry, University of Queensland, 4067 Brisbane, Australia;(2) Division of Biology, California Institute of Technology, Pasadena, CA, USA;(3) Department of Microbiology, University of Florida College of Medicine, 32610 Gainsville, FL, USA;(4) Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, 32610 Gainsville, FL, USA;(5) Department of Biochemistry, University of Queensland, Q. 4067 Brisbane, Australia |
| Abstract: | Cloned genomic DNA for human histone H1, H3 and H4 genes has been used to determine the effects of  -radiation on histone mRNA levels and synthesis in ataxia-telangiectasia cells. Synthesis of histone mRNA was determined in cells synchronized with aphidicolin. Effects of irradiation on DNA synthesis and passage through S phase were also monitored. Irradiation was found to slow the passage of control cells through the cell cycle but had no effect on progression of ataxia-telangiectasia cells. H1 and core histone mRNA synthesis was inhibited by radiation in two control cell lines after release from aphidicolin block. No inhibition was observed in one ataxia-telangiectasia cell line and a small degree of inhibition in a second. An increased level of mRNA was observed in both irradiated control and ataxia-telangiectasia cells at 5–7 h post-irradiation compared to unirradiated cells. Similar results were obtained in log phase cells. These results demonstrate that histone mRNA synthesis is radioresistant in ataxia-telangiectasia cells and is coupled to radioresistant DNA synthesis in these cells. |
| Keywords: | ataxia-telangiectasia |
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