Production of virus resistant and insect tolerant transgenic tobacco plants |
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| Authors: | Xiao-you Liang Yu-xian Zhu Jing-jiu Mi Zhang-liang Chen |
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| Institution: | (1) The National Laboratory of Protein Engineering and Plant genetic Engineering, Peking University, 100871 Beijing, China;(2) Laboratory of Molecular Genetics, College of Biology, Beijing Agricultural University, 100094 Beijing, China |
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| Abstract: | The cucumber mosaic virus coat protein (CMV-CP) gene and a modified Bacillus thuringiensis  -endotoxin (Bt toxin) gene were cloned into plant expression vector pE3. Tobacco (Nicotiana tabacum cv. G28) leaf discs were transformed with Agrobacterium tumefaciens A12 carrying recombinant pE14. Transgenic r0 and R1 tobacco plants expressing CMV-CP and Bt toxin genes were protected from CMV infection as well as feeding damage of Manduca Sexta (tobacco hornworm) larvae. These results demonstrate that it is feasible to breed new cultivars with multiple resistances via genetic engineering.Abbreviations CMV
cucumber mosaic virus
- Bt toxin
Bacillus thuringiensis -endotoxin
- CaMV
cauliflower mosaic virus
- NOS
nopaline synthase
- Kan
Kanamycin
- Spe
spectinomycin
- Carb
Carbenicillin |
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