Isolation and characterization of a generalized transducing phage for Pseudomonas aeruginosa strains PAO1 and PA14 |
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Authors: | Budzik Jonathan M Rosche William A Rietsch Arne O'Toole George A |
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Affiliation: | Dartmouth College, Hanover, New Hampshire 03755, USA. |
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Abstract: | A temperate, type IV pilus-dependent, double-stranded DNA bacteriophage named DMS3 was isolated from a clinical strain of Pseudomonas aeruginosa. A clear-plaque variant of this bacteriophage was isolated. DMS3 is capable of mediating generalized transduction within and between P. aeruginosa strains PA14 and PAO1, thus providing a useful tool for the genetic analysis of P. aeruginosa. |
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