Lipid composition and effect of amphotericin B on yeast cells of Paracoccidioides brasiliensis |
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Authors: | Júnia Scares Hamdan Maria Aparecida de Resende |
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Affiliation: | (1) Departamento de Microbiologia, Institute de Ciências Biológicas, Universidade Federal de Minas Gerais, Caixa Postal 2486, BR-30.161 Belo Horizonte - Minas Gerais, Brazil |
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Abstract: | Yeast cells of Paracoccidioides brasiliensis strain SN, were obtained for analysis of lipid composition. Total lipids, phospholipids, sterols, and qualitative sterols and fatty acid composition were determined. Such analysis were made on cells cultured in the presence or absence of amphotericin B and on non proliferating cell suspensions exposed to the antibiotic. Marked alterations in lipid contents were observed in this different conditions. The major alterations were the reduction of total lipids, sterols, and palmitoleic acid in both, proliferating and non proliferating antibiotic exposed cells. The effect of amphotericin B was evaluated also in terms of viability and release of intracellular substances, at different times of exposure. The minimal inhibitory concentration (MIC) determined for that strain of this fungus was 0.2 g/mL. |
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