ROCK-II-induced membrane blebbing and chromatin condensation require actin cytoskeleton |
| |
Authors: | Song Yuhong Hoang Bao Q Chang David D |
| |
Institution: | Department of Medicine, Microbiology, Immunology, and Molecular Genetics, UCLA School of Medicine, Los Angeles, California 900095-1678, USA. |
| |
Abstract: | Ectopic expression of ROCK II (Rho kinase II or ROKalpha), an effector of Rho GTPase, induces membrane blebbing and chromatin condensation. ROCK II can induce membrane blebbing in the presence of the caspase inhibitor z-VAD-fmk or in caspase-3-deficient MCF-7 cells, indicating that the activation of caspases is not required. ROCK-II-induced membrane blebbing, however, is reversed by the myosin light chain kinase inhibitor ML-7 or cytochalasin D. In addition, the expression of a constitutively activated form of cofilin (S3A-cofilin) suppresses both membrane blebbing and chromatin condensation in ROCK II expressing cells. These findings suggest that the activation of actin-myosin contractility is responsible for membrane blebbing and chromatin condensation and implicate ROCK II as a potential mediator of the morphological changes associated with apoptosis. |
| |
Keywords: | Rho GTPase apoptosis tetracycline-inducible expression actin filaments cofilin ML-7 caspase-3 z-VAD-fmk MCF-7 |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|