Extraction and isolation of mRNA from adult articular cartilage. |
| |
| Authors: | M E Adams D Q Huang L Y Yao L J Sandell |
| |
| Institution: | Department of Medicine, University of Calgary, Canada. |
| |
| Abstract: | We have developed a method to isolate RNA in high yield from adult articular cartilage. Homogenization of the articular cartilage with a freezer mill, extraction with 4 M guanidinium isothiocyanate/acid-phenol, and ultracentrifugation in cesium trifluoroacetate was found to be an effective and practical method for isolating a high yield of intact RNA from adult canine articular cartilage. The total RNA was suitable for Northern blot analysis. The mRNA that could then be isolated by oligo-dT affinity chromatography was found to be a suitable substrate for in vitro translation, for making a cDNA library, and for PCR amplification. |
| |
| Keywords: | |
|
|
