| 两种用于测定A/C群脑膜炎球菌多糖疫苗中乳糖含量方法的比较分析 |
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| 引用本文： | 冯潇,赵新华,傅元欣,马庆华,魏然,高雪军. 两种用于测定A/C群脑膜炎球菌多糖疫苗中乳糖含量方法的比较分析[J]. 微生物学免疫学进展, 2010, 38(4): 32-38 |
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| 作者姓名： | 冯潇 赵新华 傅元欣 马庆华 魏然 高雪军 |
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| 作者单位： | 兰州生物制品研究所,兰州730046 |
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| 摘 要： | 为探索一个灵敏、简便、快速的定量检测A/C群脑膜炎球菌多糖疫苗中乳糖含量的方法,对两种化学法进行了分析和比较。两种化学方法分别采用间苯二酚显色法和苦味酸显色法。间苯二酚显色法为套用唾液酸测定法,苦味酸显色法是对乳制品中乳糖含量检测方法的改良。两种方法的相关系数均大于0.99,线性良好。用间苯二酚法测定样品的回收试验检测结果平均值和变异系数分别为100.4%和3.09%,用苦味酸法测定样品的回收试验检测平均值和变异系数分别为99.9%和1.43%。这两种方法可以初步应用于疫苗中乳糖含量检测。
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| 关 键 词： | 疫苗 乳糖 化学检测 |
Comparison of methods for detecting lactose content in meningococcal polysaccharide vaccine |
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| Affiliation: | FENG Xiao,ZHAO Xing-hua,FU Yuan-xin,et al.(Lanzhou Institute of Biological Products,Lanzhou 730046,China) |
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| Abstract: | In order to explore a sensitive,simple and rapid quantitative detection method of the lactose content in group A and C meningococcal polysaccharide vaccine,two kinds of chemical methods,the resorcinol method and picric acid method,were analyzed.The resorcinol method is used for the detection of sialic acid,the picric acid method is an improved method of the lactose detection in the dairy products.The results showed that their correlative coefficients of those two methods are all greater than 0.99.The mean value of recovery test results and CV of resorcinol method are 100.4% and 3.09% respectively,whereas these parameters are 99.9% and 1.43% respectively in the case of using picric acid method.Both methods can be applied to detect the lactose content in vaccines. |
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| Keywords: | Vaccine Lactose Chemical detection |
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