Fed-batch cultivation for the production of clavulanic acid by an immobilized Streptomyces clavuligerus mutant |
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Authors: | Kim Il-Chul Kim Chang-Ho Hong Suk-In Kim Seung-Wook |
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Affiliation: | (1) Department of Chemical Engineering, Korea University, 1, Anam-dong, Sungbuk-ku, Seoul, 136-701, Republic of Korea;(2) Bioscience and Biotechnology, Korea–UK Co-operation Centre, Cambridge, England |
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Abstract: | In order to obtain high productivity of clavulanic acid, a newly-introduced carrier, polyurethane pellet (PUP) Z97-020 was used for the immobilization process. In a stirred-tank bioreactor, batch cultivation by Streptomyces clavuligerus KK immobilized on PUP Z97-020 gave about 3100 mg of clavulanic acid per litre, representing an increase of 200% in productivity compared with that by fed-batch cultivation of free cells (1500 mg/l). However, the clavulanic acid produced rapidly decomposed due to the pH change during batch cultivation. Fed-batch cultivation by immobilized S. clavuligerus KK gave an excellent level of clavulanic acid up to 3250 mg/l, a productivity increase of 220% compared with that by fed-batch cultivation of free cells. These results suggest that immobilization with PUP Z97-020 is a more effective process for the production of clavulanic acid and that the maintenance of pH by fed-batch cultivation with glycerol as a limiting substrate prevents the clavulanic acid from decomposing during the fermentation. |
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Keywords: | Clavulanic acid fed-batch cultivation immobilization Streptomyces clavuligerus KK |
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