Nature of the EF G-dependent binding of 3H-GMPPCP to ribosomes

The EF G-dependent binding of ³H-GMPPCP to ribosomes was studied. The binding was dependent on temperature. Methanol, but not fusidic acid, stimulated the binding at 0°C to the level of that at 37°C. Kinetic studies of the binding suggested that two EF G were involved in the binding, and that one of the two of EF G could be replaced with fusidic acid.