Somatic embryogenesis in Canary Island date palm |
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Authors: | Huong Le Thi Lan Baiocco Michela Huy Bao Phan Mezzetti Bruno Santilocchi Rodolfo Rosati Pasquale |
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Affiliation: | (1) Istituto Sperimentale per la Frutticoltura, Ciampino Aeroporto, 00040 Roma, Italy; requests for offprints. Tel.;(2) Present address: Dipartimento di Biotecnologie, Università di Milano, Italy |
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Abstract: | Shoot regeneration was obtained from leaves of in vitro cultures of wild pear genotypes. The highest regeneration rates, ranging from 40% to 64%, depending on the genotype, were obtained using leaves wounded by three cuts transversely to the mid-rib, a Quoirin and Lepoivre macro-salt composition, 250 mg l-1 cefotaxime and maintaining the explants in darkness for the first 30 days (induction phase), then transferring them to an auxin-free medium in light (expression phase). A concentration of 8.8 μM BA induced the highest number of explants to produce adventitious shoots. TDZ was less effective than BA and induced hyperhydricity in regenerated shoots. The histological studies revealed that the regenerated shoots originated mainly from callus formed by epidermal and sub-epidermal cells and by cells of the vascular tissue. The regenerated shoots were micropropagated, rooted and transplanted to the soil. This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | cefotaxime histology leaf explant organogenesis pear Pyrus communis var pyraster L. tissue culture |
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