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Interaction of rat liver microsomes containing saturated or unsaturated fatty acids with fatty acid binding protein: Peroxidation effect
Authors:A Catalá  Cesar Arcemis  A Cerruti
Institution:(1) Present address: Consejo Nacional de Investigaciones Cientificas y Técnicas de la Republica Argentina, Argentina;(2) Cátedra de Bioquímica, Sección Radioisótopos, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CC 296, 1900 La Plata, Argentina
Abstract:In the studies described here rat liver microsomes containing labeled palmitic, stearic, oleic or linoleic acids were incubated with fatty acid binding protein (FABP) and the rate of removal of14C-labeled fatty acids from the membrane by the soluble protein was measured using a model system. More unsaturated than saturated fatty acids were removed from native liver microsomes incubated with similar amounts of FABP. Thein vitro peroxidation of microsomal membranes mediated by ascorbate-Fe++, modified its fatty acid composition with a considerable decrease of the peroxidizability index. These changes in the microsomes facilitated the removal of oleic and linoeic acids by FABP, but the removal of palmitic and stearic acids was not modified. This effect is proposed to result from a perturbation of membrane structure following peroxidation with release of free fatty acids from susceptible domains.Abbreviations BSA bovine serum albumin - FABP fatty acid binding protein
Keywords:fatty acids  microsomes  peroxidation  chemiluminescence  fatty acid binding protein
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