Crystallization and some properties of cryptocytochrome c from aerobically-grown Pseudomonas denitrificans

1. 1. Analyses of cytochrome types in intact cells of aerobically-and anaerobically-grownPs. denitrificans indicated a higherratio of cytochrome c to cytochrome b in the former than inthe latter.
2. 2. Anaerobically-grown cells contained about twotimes as muchcryptocytochrome c as did aerobically-grown cells.
3. 3. Crystalline cryptocytochrome c obtained from the solublefraction of cell-free extracts of aerobically-grown cells manifestedthe same properties as cryptocytochrome c from anaerobically-growncells, i. e., absorption maxima, autooxidizability, redox potential,molecular weight, haem content, etc.
4. 4. Cryptocytochrome cwas reversibly converted to a true haemochrometype spectrumby alcohols, detergents, carboxylic acid salts,guanidine saltor high pH values.

(Received December 16, 1968; )