补糖和/或刺五加对大鼠运动后骨骼肌细胞AMPK蛋白表达的影响

目的:研究补糖和刺五加对大鼠运动后骨骼肌细胞的AMP激活蛋白激酶(AMPK)蛋白表达的影响及其恢复期的时相性变化。方法:128只SD大鼠大鼠随机分为训练对照组(C组)、训练补糖组(G组)、训练补刺五加皂甙组(A组)和训练补糖补刺五加皂甙组(GA组)四大组,补糖和刺五加均在运动后0.5 h内灌胃给予。根据运动前和运动后不同时间(0 h,4 h,12 h)采样,共分为16小组(n=8)。采用Western blot方法分析骨骼肌的AMPK蛋白含量。结果:①运动后骨骼肌的AMPK蛋白表达量上调,运动后即刻最高(209.23±21.32),随后逐渐恢复;②补药显著地提高了机体在消耗糖原运动后即刻和4 h后的股四头肌AMPK蛋白含量(225.11±20.58和186.31±15.26vs195.19±13.31和157.11±16.43),运动后12 h两组间没有差异;③补糖对骨骼肌AMPK的蛋白表达量的影响没有统计学意义;④补糖同时补药可提高运动后即刻和4 h后的股四头肌AMPK蛋白含量(217.96±19.25和191.86±14.69),但是运动后12h反而低于对照组(121.89±15.23vs137.92±16.01)。结论:运动可激活骨骼肌细胞AMPK,补充刺五加皂甙可上调运动后的AMPK蛋白表达,补糖则没有影响。

The effects of exercise and glucose and/or acanthopanacis senticosi after workout on AMPK in muscle cell of rat

Objective: To explore the effect of glucose and/or acanthopanacis senticosi administration supplement on AMP-activated protein kinase(AMPK) activation and its change in different phase after exercise in muscle cell of rat.Methods: 128 rats were divided into exercise control groups(C groups),exercise and glucose administration groups(G groups),exercise and acanthopanacis senticosi administration groups(A groups),exercise and glucose and acanthopanacis senticosi administration groups(GA groups).The glucose and acanthopanacis senticosi supplement were completed by intragastric administration in half hour after exercise.All rats were killed in different designed time points before or after glycogen depletion exercise(0 h,4 h and 12 h respectively) and finally divided into 16 groups(n=8).The values of AMPK in soleus muscle were analyzed by Western blot.Results: ①After exercise,the protein content of AMPK quickly increased and reached the peak(209.23±21.32) then gradually decreased.②Acanthopanacis senticosi administration markedly increased the protein content of AMPK in the 0 h and 4 h points after glycogen consumption training(225.11±20.58 and 186.31±15.26 vs 195.19±13.31 and 157.11±16.43) without any difference after 12 h. ③Glucose administration had no significant effect on AMPK activation.④Both glycogen and acanthopanacis senticosi were suppllied simultaneously that had enhanced the AMPK content in 4 h and 12 h point(217.96±19.25 and 191.86±14.69).However,the AMPK content in GA group was lower than that in the C groups at 12 h point(121.89±15.23 vs 137.92±16.01).Conclusion: Exercise could markedly activate the AMPK protein in muscle cell and acanthopanacis senticosi administration augmented such activation.Glucose administration had no significant effect on AMPK activation.