旋转刺激对大鼠七氟烷麻醉敏感性的影响

目的:探讨航海刺激对大鼠七氟烷麻醉敏感性的影响,为海上环境下七氟烷的合理使用提供依据。方法:以大鼠条件性厌食症作为判断指标,采用仿制Crampton旋转刺激器制作大鼠运动病模型。①60只SD大鼠随机分为对照组(n=15)和旋转组(n=45),观测2组大鼠的活动和行为变化、达到睡眠和麻醉状态所需七氟烷浓度以及麻醉苏醒时间。②32只SD大鼠随机分为四组(n=8):对照(Ⅰ)组、旋转(Ⅱ)组、麻醉(Ⅲ)组和旋转后再麻醉(Ⅳ)组,分别检测4组大鼠之大脑皮层、下丘脑和海马组织的乙酰胆碱、去甲肾上腺素、г-氨基丁酸及谷氨酸含量。结果:对照组大鼠达到睡眠和麻醉状态所需七氟烷浓度分别为1.74%±0.05%和3.54%±0.05%,旋转组分别为1.51%±0.06%和3.14%±0.08%,旋转组大鼠达到睡眠和麻醉状态所需七氟烷浓度明显降低(P<0.01)。神经递质变化以Ⅲ组大鼠各递质含量显著降低为其主要特点,在大脑皮层和海马组织中表现尤为明显(P<0.01)。Ⅱ组大鼠大脑皮层Ach显著升高,海马组织NE、GABA明显降低。Ⅳ组变化趋势更接近于Ⅱ组,即以旋转刺激的影响更为明显。结论:模拟航海刺激可明显增加大鼠对七氟烷麻醉的敏感性。

Effect of rotation stimulation on the anesthetic sensitivity of sevoflurane in rats

Objective: To explore the effect of simulated navigation stimulation on the anesthetic sensitivity of sevoflurane in rats,so as to provide basis for rational using sevoflurane during navigation.Methods: SD rats were stimulated by Crampton model and the conditioned taste aversion(CTA) was regarded as criterion of motion sickness.① 60 rats were randomly divided into control(n=15) and rotation group(n=45).The changes of behavior and autonomic activity,sevoflurane concentration achieved sleep and anesthesia states,and the revitalization time were observed in two group rats.② 32 rats were randomly divided into control(I),rotation(II),anesthesia(III) and rotation plus anesthesia(IV) group(n=8).The acetylcholine(Ach),norepinephrine(NE),г-aminobutyric acid(GABA),glutamic acid(Glu) of brain cortex,thalamus and hippocampus were determined respectively in the four group rats.Results: In control group,the sevoflurane concentration achieved sleep and anesthesia states were 1.74%±0.05% and 3.54%±0.05% respectively,but,those concentrations were 1.51%±0.06% and 3.14%±0.08% in rotation group.There were lower significantly in rotation group than those in control group(P<0.01).It was a major characteristic that all of the neurotransmitters were reduced significantly in Ⅲ group,this was even more in brain cortex and thalamus(P<0.01).In Ⅱ group,Ach was upward in brain cortex,NE and GABA were reduced in hippocampus obviously.The change tendency of neurotransmitters in Ⅳ group was more close to Ⅱ group,that was,the effect of rotation stimulation was more obvious.Conclusion: The anesthetic sensitivity of sevoflurane could be obvious increased in rats simulated navigation stimulation.