Long term plant regeneratin from callus cultures of haploid wheat |
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Authors: | C. C. Chu Y. X. Wang J. L. Sang |
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Affiliation: | (1) Department of Forestry, University of Illinois at Urbana-Champaign, 61801 Urbana-Champaign, IL, USA;(2) Department of Horticulture, University of Illinois at Urbana-Champaign, 61801 Urbana-Champaign, IL, USA;(3) Department of Plant Pathology, University of Illinois, N519 Turner Hall, 1102 South Goodwin Ave., 61801 Urbana, II, USA |
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Abstract: | A one-step method to rescue immature embryos of eastern cottonwood (Populus deltoides Bartr.) is described. Plantlets developed from 83% of 25-day-old embryos grown in shaken culture on Murashige and Skoog (MS) liquid medium with 2.2 m indole-3-acetic acid (IAA) and from 86% of embryos not supplemented with IAA. In contrast, when the MS medium was solidified with 0.8% agar, plantlets developed from 25% of 25-day-old embryos cultured on medium supplemented with IAA and from 28% of embryos in medium not supplemented with IAA. Eighty eight percent of all plantlets survived a gradual acclimitization to peat plugs in a greenhouse. The one-step liquid-culture method is an effective means of rescuing immature embryos by ovule culture from excised artificially-pollinated female branches in our cottonwood breeding program. |
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Keywords: | Embryo culture Embryo rescue Ovule culture Populus deltoides Bartr. |
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