Fluorescent derivatives of prostaglandins and thromboxanes for liquid chromatography |
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| Authors: | John Turk Stephen J. Weiss J.E. Davis Philip Needleman |
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| Affiliation: | Division of Laboratory Medicine (Department of Pathology) and Department of Pharmacology, Barnes Hospital and the Washington University School of Medicine, 4960 Audubon, St. Louis, MO, USA |
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| Abstract: | Fluorescent esters of the prostablandins D2, E2, F2α, and 6-keto-F1α and of thromboxane B2 have been prepared using the reagent 4-bromomethyl-7-methoxycoumarin. All of these derivatives can be separated in a single run either by thin-layer or high-performance liquid chromatography (TLC or HPLC). As little as 20 ng of PGE2 can be detected after derivatization and HPLC analysis. Identification of thromboxane B2 produced by human platelets and of 6-keto-PG F1α produced by bovine aortic microsomes has been achieved with this method. |
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