| cre／lox P系统介导的基因重组研究进展 |
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| 引用本文： | 屈彦纯,邓昌彦,刘建忠,刘桂兰. cre／lox P系统介导的基因重组研究进展[J]. 生物技术通报, 2001, 0(5): 8-12 |
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| 作者姓名： | 屈彦纯 邓昌彦 刘建忠 刘桂兰 |
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| 作者单位： | 华中农业大学畜牧兽医学院 |
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| 摘 要： | 由于具有时间，组织和位点特异性,cre重组酶介导的DNA重组已成为基因靶位操作的一个重要工具，概述了cre/loxP系统的作用特点，cre重组酶的结构和重组机制，表达载体构建及重组个体检测等方面的问题，重点介绍了cre/lox P系统在基因重组研究中的应用及近来的发展与成就。
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| 关 键 词： | cre／lox P系统 基因重组 基因靶位操作 介导 |
Recent Progress in Research on Gene Recombination Mediated bycre/lox P System |
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| Qu Yanchun Deng Changyan Liu Jianzhong Liu Guilan. Recent Progress in Research on Gene Recombination Mediated bycre/lox P System[J]. Biotechnology Bulletin, 2001, 0(5): 8-12 |
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| Authors: | Qu Yanchun Deng Changyan Liu Jianzhong Liu Guilan |
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| Abstract: | DNA Recombination mediated by cre recombinase has become an important tool for gene targeting because it is time-specific, tissue-specific and site-specific. In this paper, the recombinent characteristics of the cre/loxP system,the structure of cre recombinase and its recombination mechanism, the construction of expression vector and the detection of the result of recombination in cre/loxP system is reviewed.The ultilization of cre/lox P system and its recent development and achivements are also introduced as an important aspect. |
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| Keywords: | cre/lox P system DNA Recombination gene targeting |
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