Lipid bilayer tethered inside a nanoporous support: a solid-state nuclear magnetic resonance investigation |
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Authors: | Wattraint Olivier Arnold Alexandre Auger Michèle Bourdillon Christian Sarazin Catherine |
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Affiliation: | Unité de Génie Enzymatique et Cellulaire, UMR 6022 du CNRS, Université de Picardie Jules Verne, 33 rue Saint-Leu, 80039 Amiens, France. |
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Abstract: | (31)P and (1)H solid-state nuclear magnetic resonance (NMR) experiments have been designed with the aim of studying directly the formation of supported bilayers tethered inside nanoporous aluminum oxide supports as a model of biomimetic membranes. The static and magic angle spinning (31)P NMR spectra of the supported bilayers have been compared with the experimental and simulated spectra of a simpler model with cylindrical geometry, namely a phospholipid bilayer adsorbed on an oriented polymer sheet. The broadening observed for the nanoporous model is most likely due to the presence of paramagnetic ions in the aluminum oxide. A phospholipid lateral diffusion coefficient of (2.8 +/- 0.4) x 10(-8) cm(2)/s has been measured for the tethered bilayer on a spherical support, indicating a good fluidity as compared with adsorbed membrane models. |
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Keywords: | Tethered phospholipid bilayers Nanoporous and polymer support Static and MAS solid-state NMR |
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