| 糖胺聚糖降解菌株的筛选及鉴定 |
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| 引用本文： | 雷曦,周峻沛,黄遵锡,丁琳,张蕊. 糖胺聚糖降解菌株的筛选及鉴定[J]. 微生物学杂志, 2020, 0(3): 22-27 |
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| 作者姓名： | 雷曦 周峻沛 黄遵锡 丁琳 张蕊 |
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| 作者单位： | 云南师范大学生命科学学院，云南 昆明 650500;1.云南师范大学生命科学学院，云南 昆明 650500;2.生物能源持续开发利用教育部工程中心，云南 昆明 650500；3.云南省生物质能与环境生物技术重点实验室，云南 昆明 650500；4.云南师范大学 酶工程重点实验室，云南 昆明 650500 |
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| 基金项目： | 国家重点研发计划项目（2017YFB0308401）；国家自然科学基金项目(31460694) |
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| 摘 要： | 以土壤为材料,用透明质酸和硫酸软骨素为唯一碳源富集分离菌株,通过BSA-乙酸平板显色法及比色定糖法进行筛选。从80份土壤中筛选出13株糖胺聚糖降解活性的菌株并对其进行了16S rDNA测序鉴定。结果表明,筛选到13株糖胺聚糖降解菌株均具有透明质酸酶和硫酸软骨素酶活性;获得8株尚未报导过的产糖胺聚糖降解酶活性菌株。本研究为开发新型的糖胺聚糖降解酶提供参考。
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| 关 键 词： | 糖胺聚糖降解酶（Glycosaminoglycan degrading enzyme）；透明质酸；硫酸软骨素；菌株筛选；16S rDNA |
Screening and Identification of Glycosaminoglycan Degradation Strains |
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| LEI Xi,ZHOU Jun-pei,HUANG Zun-xi,DING Lin,ZHANG Rui. Screening and Identification of Glycosaminoglycan Degradation Strains[J]. Journal of Microbiology, 2020, 0(3): 22-27 |
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| Authors: | LEI Xi ZHOU Jun-pei HUANG Zun-xi DING Lin ZHANG Rui |
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| Abstract: | In this study, soil was taken as material adopting hyaluronic acid and cartilage sulfate as the sole carbon source to enrich and isolate strains. And glycosaminoglycan degradation strains were screened by BSA-acetic acid plate coloration method and colorimetric method. 13 strains with glycosaminoglycan degradation activity were screened from 80 soil samples and sequenced by 16S rDNA. The results showed that glycosaminoglycan degradation strains had both activities of hyaluronidase and chondroitinase. The obtained 8 novel strains among them have not been reported to produce glycosaminoglycan degrading enzyme so far. The study provided a foundation to develop novel glycosaminoglycan degradation enzymes. |
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| Keywords: | Glycosaminoglycan degrading enzyme hyaluronic acid chondroitin sulfate strain screening 16S rDNA |
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