Terpinen-4-ol and carvacrol affect multi-species biofilm composition |
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| Authors: | Patricia Milagros Maquera Huacho Esteban Rodriguez Herrero Tim Verspecht Martine Pauwels Elcio Marcantonio Jr Denise Madalena Palomari Spolidorio |
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| Institution: | 1. Disaster Prevention and Water Environment Research Center, National Chiao Tung University , 1001 University Road, Hsinchu, 300, Taiwan;2. Institute of Environmental Engineering, National Chiao Tung University , 1001 University Road, Hsinchu, 300, Taiwan;3. Department of BioEnvironmental Engineering and R&4. D Center for Membrane Technology , Chung Yuan Christian University , 200 Chung Pei Road, Chunli, 320, Taiwan |
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| Abstract: | The aim of this study was to investigate the cytotoxic activity and inhibitory effect of terpinen-4-ol (T4ol) and carvacrol against single- and multi-species biofilms. The toxicity of each compound was tested on oral keratinocytes and evaluated by XTT assay. Inhibition and eradication of single-species biofilms were analyzed by crystal violet assay and the effect on multi-species biofilm composition was evaluated by qPCR. T4ol and carvacrol did not affect the epithelial cell viability, in contrast to chlorhexidine, which showed a high cytotoxic effect. Inhibition and eradication of single-species biofilms treated with T4ol and carvacrol were observed. The same inhibitory effect was observed for multi-species biofilms, especially on periodontal pathogens. In conclusion, specific concentrations of T4ol and carvacrol without toxicity towards the epithelial cells reduced the numbers of periodontal pathogens in single- and multi-species biofilms. |
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| Keywords: | Terpinen-4-ol carvacrol peri-implantitis periodontal disease multi-species biofilm |
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