Separation of biological particles by centrifugal elutriation |
| |
| Authors: | C R McEwen R W Stallard E T Juhos |
| |
| Affiliation: | 1. Beijing Agro-Biotechnology Research Center, Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, PR China;2. Jilin Originwater Technology Co., LTD, Changchun 130012, PR China;3. Key Laboratory of Urban Agriculture (North), Ministry of Agriculture, Beijing 100097, PR China;1. Department of Environmental Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 100083, China;2. Department of Environmental Science and Engineering, School of Space and Environment, Beihang University, Beijing 10191, China;3. Beijing Key Laboratory on Resource-Oriented Treatment of Industrial Pollutants, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing 10083, China;1. Escola d’Enginyeria d’Igualada, Universitat Politecnica de Catalunya Barcelona Tech (EEI-UPC), Av. Pla de la Massa, 8, Igualada 08700, Spain;2. Centre Català del Plàstic (CCP), Universitat Politecnica de Catalunya Barcelona Tech (EEBE-UPC), C/Colom, 114, Terrassa 08222, Spain;1. Key Laboratory of Groundwater Circulation and Evolution (China University of Geosciences Beijing), Ministry of Education, Beijing, 100083, China;2. School of Water Resources and Environment, China University of Geosciences (Beijing), No. 29 Xueyuan Road, Haidian District, Beijing, 100083, China;3. Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan;4. School of Environmental Engineering, Henan University of Technology, Zhengzhou, 450001, China |
| |
| Abstract: | Centrifugal elutriation of biological particles has been performed in specially built rotors in a standard preparative centrifuge. Polystyrene microspheres, yeast cells, and algae cells have been separated by size or density in sodium chloride elutriating media; whole blood and leukocyte-enriched blood have been elutriated in a buffered salt solution with added glucose for leukocyte metabolism. By elutriation of whole blood, the ratio of erythrocytes to leukocytes was reduced from 687:1 in the original cell population to 3.9:1 in the population retained in the separation chamber. By elutriation of leukocyte-enriched blood, lymphocytes were washed away, yielding a granulocyte population of from 89 to 95%. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
