Oxidative enzymes in the pancreatic islets of normal and obese-hyperglycemic mice |
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Authors: | Bo Hellman Claes Hellerström |
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Institution: | (1) Histological Department, University of Uppsala, Uppsala, Sweden |
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Abstract: | Summary Histochemical data are presented concerning distributions of succinic dehydrogenase (SD), lactic dehydrogenase (LD), diphosphopyridine nucleotide diaphorase (DPND), triphosphopyridine nucleotide diaphorase (TPND) and glucose-6-phosphate dehydrogenase (G-6-PD) in the pancreas from the American variety of obese-hyperglycemic mice (AO-mice) and their lean litter mates (AN-mice).A high LD activity was found in the exocrine parenchyma, while the reaction in the islet tissue and the duct epithelium was only weak. A considerable reaction for DPND was noted throughout the pancreas. SD activity was slightly more pronounced in the acinar tissue and duct epithelium as compared to the islet tissue, where only a moderate activity appeared. Strong reactions for TPND and G-6-PD were found in the islet cells and duct epithelium, while the activity in the exocrine parenchyma was less pronounced. The hyperactive islet B cells in the AO-mice showed no obvious differences in enzyme activity and distribution compared to that of the AN-mice. The enzyme pattern of the A cells could not be clearly distinguished from that in the B cells.The results suggest the existence at least in the B cells of the mice islet tissue of an active hexosemonophosphate shunt. The probable significance of the hexosemonophosphate shunt for insulin synthesis is briefly discussed.The following abbreviations are used DPN
Diphosphopyridine nucleotide
- DPND
Diphosphopyridine nucleotide diaphorase
- DPNH
Diphosphopyridine nucleotide, reduced form
- EM
Embden-Meyerhof
- G-6-PD
Glucose-6-phosphate dehydrogenase
- HMP
Hexose monophosphate
- LD
Lactic dehydrogenase
- MTT
3,5-diphenyl-2-(4,5-dimethyl-thiazol-2-yl) tetrazolium bromide
- Nitro-BT
2,2-di-p-nitrophenyl-5,5-diphenyl-3,3-(3,3-dimethoxy-4,4-biphenylene)-ditetrazolium chloride
- PVP
Polyvinyl pyrrolidone (M. W. 11 000)
- SD
Succinic dehydrogenase
- TPN
Triphosphopyridine nucleotide
- TPND
Triphosphopyridine nucleotide diaphorase
- TPNH
Triphosphopyridine nucleotide, reduced form |
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