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Regeneration of transgenic tamarillo plants |
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| Authors: | Ross G. Atkinson Richard C. Gardner |
| Affiliation: | (1) Centre for Gene Technology, School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand;(2) The Horticulture and Food Research Institute of New Zealand Ltd, Private Bag 92169, Mt Albert, Auckland, New Zealand |
| Abstract: | Media were developed to regenerate shoots from leaf pieces of tamarillo (Cyphomandra betacea (Cav.) Sendtner). Shoots were derived via organogenesis and could be easily rooted and transferred to the growth chamber. Transgenic tamarillo plants were produced using the binary vector pKIWI110 in the avirulent Agrobacterium strain LBA4404. All transgenic plants were kanamycin resistant and some plants expressed the  D-glucuronidase (gusA) reporter gene and were chlorsulfuron resistant. Molecular evidence for transformation was obtained using PCR (polymerase chain reaction) and Southern hybridization. Inheritance of the transgenic phenotypes was demonstrated in seedling progeny. |
| Keywords: | Agrobacterium-mediated transformation Cyphomandra betacea pKIWI110 Regeneration Tamarillo |
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