Immobilization of enzymes on spheron: 1. Trypsin and glucoamylase by the titanium-chelation method |
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Authors: | CJ Gray CM Lee SA Barker |
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Institution: | Department of Chemistry, University of Birmingham, P.O. Box 363, Edgbaston, Birmingham B15 2TT, UK |
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Abstract: | In a preliminary study, trypsin (EC 3.4.21.4) and glucoamylase (exo-1,4-α-d-glucosidase, 1,4-α-d-glucan glucohydrolase, EC 3.2.1.3) were immobilized on Spheron by the titanium-chelation method. The activity of trypsin immobilized on Spheron P100 000 was higher against tosyl-l-arginine 4-nitroanilide than against casein. The variation in the specific activities of glucoamylase immobilized on Spherons of different porosities to wards substrates of different molecular weights was examined. |
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Keywords: | Titanium chelation enzyme immobilization trypsin EC 3 4 21 4 Spheron |
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