Factors affecting microbial growth and polysaccharide production during the fermentation of Xanthomonas campestris cultures |
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| Authors: | John F. Kennedy Peter Jones S.Alan Barker G.T. Banks |
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| Affiliation: | Research Laboratory for the Chemistry of Bioactive Carbohydrates and Proteins, Department of Chemistry, University of Birmingham, PO Box 363, Birmingham B15 2TT, UK;Dept of Biochemistry, Imperial College, South Kensington, London SW7 2AZ, UK |
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| Abstract: | Fermentations of Xanthomonas campestris have been carried out on laboratory and pilot plant scales using various organic nitrogen sources in order to test their effectiveness in polysaccharide (xanthan) production. It was discovered that high nitrogen concentrations give highest yields of crude product and result in a need for only short fermentation times to achieve maximum product formation. These products, however, have inferior solution rheology to those produced from low-nitrogen media due partly to their high concentrations of co-precipitated microbial cells and partly to differences in tertiary molecular structure. |
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| Keywords: | Fermentation xanthan rheological properties nitrogen source microbial growth |
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