Accumulation of Hydrogen Peroxide in Chloroplasts of SO2-Fumigated Spinach Leaves |
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Authors: | Tanaka Kiyoshi; Kondo Noriaki; Sugahara Kiyoshi |
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Institution: | Division of Environmental Biology, the National Institute for Environmental Studies Yatabe, Ibaraki 305, Japan |
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Abstract: | Illuminated chloroplasts isolated from SO2-fumigated spinachleaves accumulated more H2O2 than those from non-fumigated ones.This H2O2 formation was dependent on light and was inhibitedby DCMU. It also was depressed by cytochrome c and superoxidedismutase (EC 1.15.1.1
EC]
). The addition of sulfite to rupturedchloroplasts isolated from non-fumigated leaves caused an H2O2accumulation that accompanied O2 uptake. Spinach leaves losttheir catalase (EC 1.11.1.6
EC]
), ascorbate peroxidase and glutathionereductase (EC 1.6.4.2
EC]
) activities at the beginning of SO2 fumigation,when H2O2 was accumulated. These results suggest that the accumulationof H2O2 in SO2-fumigated spinach leaves is caused by the increasein O2production, the precursor for H2O2, with a sulfite-mediatedchain reaction at the reducing site of photosystem I, and byinactivation of the H2O2 scavenging system. (Received October 7, 1981; Accepted June 16, 1982) |
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