FGFR4 mRNA及蛋白在乳腺癌组织中的表达及临床意义

目的:研究乳腺癌组织与乳腺癌癌旁组织中FGFR4 mRNA及蛋白的表达及其临床意义。方法:分别以实时荧光定量RT-PCR、Western blot的方法检测52例乳腺癌组织和52例癌旁正常组织中FGFR4 mRNA和蛋白的表达,分析FGFR4 mRNA和蛋白的表达与临床病理特征的相关性。结果:在乳腺癌组织中,FGFR4 mRNA及蛋白的表达均高于在乳腺癌癌旁正常组织(P0.05),并且FGFR4的表达与患者淋巴结转移和Her-2相关,而与患者年龄、肿瘤大小、分化程度、ER和PR无明显相关性(P0.05)。结论:FGFR4 mRNA及蛋白在乳腺癌组织中表达升高,与淋巴结转移和Her-2有关,有望成为预测乳腺癌的转移和预后的参考指标之一。

Expression and Clinical Significance of FGFR4 mRNA and Protein in the Breast Cancer

ABSTRACT Objective: To study the expression and clinical significance of FGFR4 mRNA and protein in the breast cancer tissues and adjacent tissues. Methods: Real-time fluorescence quantitative RT-PCR and Western blot methods were used respectively to detect the expression of FGFR4 mRNA and protein in 52 cases of breast cancer tissues and adjacent tissues. Results: The expressions of FGFR4 mRNA and protein in the breast cancer tissues were higher than those of the adjacent tissues(p<0.05), and they were associated with the lymph node metastasis and Her-2 expression(p<0.05), but had no correlation with the age, tumor size, degree of differentiation, ER and PR of breast cancer(P>0.05). Conclusion: FGFR4 mRNA and protein expressions in the breast cancer tissue were relate to the lymph node metastasis and Her-2 expression. FGFR4 mRNA and protein were expected to be the indicatiors of breast cancer metastasis and prognosis.