SLE带状疱疹患者外周血CD4~+CD28~+和CD4~+CD25~+FoxP3~+调节性T细胞的表达及相关性研究

目的:检测系统性红斑狼疮(systemic lupus erythematosus,SLE)合并带状疱疹患者外周血CD4~+CD28~+和CD4~+CD25~+Fox P3~+调节性T细胞的表达及相关性,探讨其在SLE合并带状疱疹发病中的临床意义。方法:采用流式细胞术检测30例SLE患者、30例SLE合并带状疱疹患者及30例健康对照者外周血中CD4~+/CD8~+T淋巴细胞亚群表面CD28的表达及CD4~+CD25~+Fox P3~+Treg细胞的表达水平,并分析SLE合并带状疱疹患者外周血CD4~+CD28~+和CD4~+CD25~+Fox P3~+调节性T细胞表达的相关性。结果:SLE合并带状疱疹组患者急性期外周血CD4~+T淋巴细胞比率、绝对计数显著降低,CD4~+、CD8~+T淋巴细胞表面的CD28表达下调,CD4~+CD25~+Fox P3~+Treg细胞水平显著高于SLE组及健康对照组,SLE合并带状疱疹组患者外周血CD4~+CD25~+Fox P3~+Treg水平与CD4~+CD28~+水平成负相关(P均0.05)。结论:SLE合并带状疱疹患者CD4~+、CD8~+T细胞活化异常,CD4~+CD25~+Fox P3~+Treg细胞可能参与抑制了T细胞的活化。

The Expression and Correlation of CD4+CD28+T Cells and Treg Cells in the Peripheral Blood of Patients with Systemic Lupus Erythematosus combined with Herpes Zoster

Objective:To investigate the expression and correlation of CD28 on CD4+/CD8+T cells and Treg cells in peripheral blood of systemic lupus erythematosus (SLE) patients with acute herpes zoster and explore the significance of its change in development of SLE patients with acute herpes zoster.Methods:The expression of CD28 on CD4+/CD8+T cells and frequencies of CD4+CD25+FoxP3+Treg cells were analyzed by flow cytometry in 30 SLE patients, 30 SLE patients with acute herpes zoster and 30 healthy controls, the correlation of CD4+CD28+T cells and CD4+CD25+FoxP3+Treg cells was analyzed in SLE patients with acute herpes zoster.Results:It was observed that CD4+, CD4+CD28+, CD8+CD28+T cells were significantly lower and CD4+CD25+FoxP3+Treg cells were significantly higher and CD4+CD25+FoxP3+Treg cells were correlated negatively with CD4+CD28+T cells in the peripheral blood from SLE patients with acute herpes zoster than those in SLE Group and in healthy controls (P<0.05).Conclusion:The results demonstrate that the decline of T cellular immune function may play a key role in the pathogenesis of herpes zoster. CD4+CD25+FoxP3+Treg cells may be related to the development of SLE with acute herpes zoster.