乳腺癌细胞中HuR调控PDFC的分子机制研究 |
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引用本文: | 罗年安,陈玉宝,武晓军,董 瑞. 乳腺癌细胞中HuR调控PDFC的分子机制研究[J]. 现代生物医学进展, 2017, 17(25): 4828-4830 |
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作者姓名: | 罗年安 陈玉宝 武晓军 董 瑞 |
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作者单位: | 第四军医大学附属唐都医院普外科 陕西 西安710032;解放军第十医院 甘肃 武威 733000 |
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基金项目: | 国家自然科学基金项目(81001181);陕西省自然科学基金项目(2016JM8066) |
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摘 要: | 目的:探讨乳腺癌细胞中HuR调控PDGFC的分子机制。方法:通过软件预测分析,乳腺癌细胞中PDGFC 3'UTR的HuR结合位点;在RNA免疫共沉淀实验中,加入PDGFC刺激后检测HuR与PDGFC mRNA的相互作用;通过构建PDGFC 3'UTR五个截短体,荧光素酶报告基因实验检测HuR调控PDGFC的结合位点。结果:软件预测分析发现,PDGFC 3'UTR可能存在五个HuR结合位点;RNA免疫共沉淀实验中,当加入PDGFC刺激后,HuR与PDGFC mRNA出现免疫共沉淀,证明HuR和PDGFC之间的直接相互作用;PDGFC mRNA3'UTR报告基因系统检测显示,第2个和第4个位点可与HuR结合调控PDGFC。结论:本研究揭示了乳腺癌细胞中HuR通过与PDGFC mRNA 3'UTR结合调控PDGFC的分子机制,为乳腺癌的临床诊断和治疗提供了新的思路。
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关 键 词: | 乳腺肿瘤;HuR;PDGFC;分子机制 |
收稿时间: | 2016-12-09 |
修稿时间: | 2016-12-29 |
Molecular Mechanism of PDGFC Regulated by HuR in Breast Cancer Cells |
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Abstract: | ABSTRACT Objective: To investigate the molecular mechanism of PDGFC regulated by HuR in breast cancer cells. Methods: Through the software analysises, we first predicted the HuR-binding sites on the PDGFC 3''-UTR in breast cancer cells; An RNA-im- munoprecipitation tested the interaction of HuR with the PDGFC mRN after adding PDGFC stimulation; Luciferase experiments tested the HuR-binding sites of PDGFC regulated by HuR through structuring five truncated of PDGFC 3''UTR. Results: We found five HuR-binding sites in the 3''UTR of PDGFC by software prediction; The RNA-immunoprecipitation showed the co-immunoprecipitation of HuR and PDGFC mRN after adding PDGFC stimulation confirming the direct association of HuR with the PDGFC; Luciferase experi- ments of PDGFC mRNA 3''UTR showed that PDGFC regulated by the second and fourth HuR-binding sites. Conclusion: This study re- veals the molecular mechanism of PDGFC regulated by HuR through binding to PDGFC mRNA 3''UTR in breast cancer cells, and pro- vided rationale for the development of strategies in the clinical diagnosis and treatment for breast cancer. |
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Keywords: | Breast neoplasms HuR PDGFC Molecular mechanism |
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