脑源性神经生长因子对齿状回miR-132表达及抑制性电流的影响

目的:探讨脑源性神经生长因子(BDNF)对海马mi R-132的表达及齿状回颗粒细胞抑制性突触后电流(sIPSCs)的影响,明确BDNF对颞叶内侧癫痫(MTLE)发病机制的作用。方法:选取哈医大一院神经外科2008年4月-2010年10月手术治疗的MTLE患者12例海马组织。RT-pcr技术检测BDNF孵育后mir-132表达,脑片膜片钳技术检测BDNF对sIPSCs的影响。结果:BDNF升高了颞叶癫痫海马mi RNA-132的表达(P0.01),减弱了颗粒细胞sIPSCs的频率和幅度(P0.01)。结论:BDNF升高了海马mir-132的表达,减弱颗粒细胞sIPSCs的频率和幅度,可能对MTLE的发展有促进作用。

Effect of BDNF on The Levels of miRNA-132 and sIPSCs in the Dentate Gyrus of MTLE

ABSTRACT Objective: To study the effect of brain derived neurotrophic factor (BDNF) on the levels of miRNA-132 and sIPSCs in the dentate gyrus of patients with MTLE. Methods: The study was performed from April 2008 to October 2010. Surgically removed specimens were collected from the patients with MTLE. All patients gave written informed consent for research use of the biopsy materials. Surgically resected hippocampal were collected and immediately immersed in oxygenated ice-cold SACSF. The expression of mIR-132 by RT-PCR and sIPSCs by patch-clamp. Results: The expression of miR-132 was significantly increased in Dentate Gyrus of MTLE patients after BDNF perfusion was significantly higher than that before perfusion P<0.01). The frequency and amplitude of sIPSC were obviously lower in Dentate Gyrus of MTLE patients after BDNF perfusion was significantly higher than that before perfusion (P<0.01). Conclusion: BDNF increased the expression of miR-132 and inhibited the frequency and amplitude of sIPSCs of granule cells in the hippocampus of MTLE patients after operation, which could contribute to the development of MTLE.