男性精浆中IL-6 和sICAM-1 与免疫性不育的关系研究

目的:探究男性精浆中白细胞介素-6(IL-6)和可溶性细胞黏附分子-1(sICAM-1)与免疫性不育的关系。方法:选择2014年6月至2015年12月我院收治不育症患者189例及100例健康体检者为研究对象,根据患者精子混合抗球蛋白反应实验(MAR)结果将不育症患者分为免疫性不育组(88例)和非免疫性不育组(101例);免疫不育组患者按照精液白细胞过氧化物酶染色情况分为免疫性阳性白细胞组(WBC≥1×10~6/mL)36例和免疫性阴性白细胞阴性组(1×10~6/m L)52例,分析并比较各组间精子质量、IL-6和sICAM-1水平。结果:免疫性不育组与非免疫性不育组患者精液精子向前运动比率、存活率均低于对照组(P0.05);免疫性不育组与非免疫性不育组精液各项参数均不存在差异(P0.05);免疫性不育组患者IL-6、sICAM-1水平均高于非免疫性不育组及对照组,差异有统计学意义(P0.05);非免疫性不育组IL-6、sICAM-1水平均高于对照组,差异有统计学意义(P0.05)。相关性分析显示,精子被Ig G黏附数比例与研究对象精液IL-6、sICAM-1水平呈正相关关系(r=0.438,0.561;P0.05);免疫性阳性白细胞组患者精液IL-6与sICAM-1水平均高于免疫性阴性白细胞组(P0.05)。结论:免疫性不育症患者精子质量下降,男性精液中IL-6和sICAM-1水平表达越高,MAR阳性率越高,男性免疫性不育发生的可能性越大。

Study on the Relationship between the Level of IL-6, sICAM-1 and ImmuneInfertility in Male Seminal Plasma

Objective:To explore the relationship between the level of IL-6, sICAM-1 and immune infertility in male seminalplasma.Methods:189 cases of infertile patients and 100 cases of healthy physical examination people were selected as the research objectsfrom June 2014 to December 2015, and the infertility patients were divided into immune infertility group (88 cases) and non-immuneinfertility group (101 cases) according to the results of patient''s sperm with Mixed Agglutination Reaction (MAR); Patients in immuneinfertility group were divided into immune positive cells group (WBC is more than or equal to 1× 106/mL) (36 cases) and immunenegative leukocyte negative group (< 1× 106/mL) of 52 cases accordance to the seminal leucocyte peroxidase staining; To analysis andcompare the sperm quality, the level of IL-6 and sICAM-1 between the each groups.Results:The rate of sperm forward movement andthe survival rate of the sperm in immune infertility group and non-immune infertility group were lower than those of the control group(P<0.05); There were no differences in semen parameters between the immune infertility group and the non-immune infertility group(P>0.05); The levels of IL-6 and sICAM-1 in the immune infertility group were significant higher than those in the non-immune infertilitygroup and the control group (P<0.05); The levels of IL-6 and sICAM-1 in the non-immune infertility group were significant higher thanthose in control group (P<0.05); Correlation analysis showed that the sperm were IgG adhesion ratio were positively correlated with thelevel of IL-6, sICAM-1(r=0.438,0.561, P<0.05) among research object; The levels of IL-6 and sICAM-1 in the patients with immune positiveleukocyte were higher than those of the immune negative leukocyte group (P<0.05).Conclusion:The sperm quality of immune infertilitypatients were decreased, the higher expression of IL-6 and sICAM-1 in male semen, the higher MAR positive rate, the greater thepossibility of male immune infertility.