不同盐浓度对盐敏感性高血压肾脏纤维化及巨噬细胞浸润的影响

目的:探讨不同盐浓度对盐敏感高血压肾脏纤维化及及巨噬细胞浸润的影响。方法:将6周龄Dahl盐敏感大鼠随机分为正盐组(0.3%氯化钠)、4%氯化钠组、8%氯化钠组三组,每组15只,持续喂养8周。在8周时间点应用tail-cuff method方法监测鼠尾血压,Masson三原色法检测三组大鼠肾脏组织纤维化情况,免疫组化及Western blotting方法检测各组大鼠肾脏巨噬细胞浸润情况。结果:1)4%高盐组及8%高盐组大鼠8周时间点血压均较正盐组现在升高,8%高盐组大鼠血压较4%高盐组进一步升高。2)4%高盐组及8%高盐组大鼠8周时间点肾脏相对重量及肾脏纤维化程度较正盐组增加,8%高盐组较4%高盐组进一步增加。3)4%高盐组及8%高盐组大鼠8周时间点肾脏组织巨噬细胞浸润增加,8%高盐组较4%高盐组进一步增加。结论:盐浓度不同对盐敏感性肾脏纤维化及巨噬细胞浸润程度影响不同,盐浓度升高可加重盐敏感高血压时肾脏纤维化及及巨噬细胞浸润。

Influence of Different Dietary Salt Concentration on the Renal Fibrosis and Macrophages Infiltration in Salt Sensitive Hypertension

ABSTRACT Objective: To investigate the influence of different salt concentration on the renal fibrosis and macrophages infiltration in salt sensitive hypertension. Methods: Dahl salt sensitive rats were randomly divided into the normal salt (0.3 % nacl) group, 4 % high salt, 8 % high salt groups at six weeks continuously feeding for 8 weeks, each group contained 15 rats. Tail-cuff method was used to value rat blood pressure at 8 weeks, Masson trichromatic method was used to detect renal fibrosis of the three groups at 8 week. Immunohisto- chemistry and Western blot method were used to depict the renal macrophage infiltration at 8 week. Results: 1) The blood pressure of 4 % salt and 8% high salt group rats were significantly higher than those of the normal salt group at 8week, meanwhile the blood pressure of 8 % high salt was further increased than that of 4 % high salt group at 8 week. 2) The relative kidney weight and renal fibrosis of 4 % salt and 8 % high salt group rats were obviously higher than that of normal salt group at 8week, meanwhile the relative kidney weight and renal fibrosis of 8 % high salt were further increased than those of 4 % high salt group at 8 week. 3) The macrophage infiltration of 4 % salt and 8% high salt group rats were higher than that of the normal salt group at 8week, meanwhile the macrophage infiltration of 8 % high salt was further increased than that of 4 % high salt group at 8 week. Conclusion: Different high salt concentrations had different ef- fect on the renal fibrosis and macrophage infiltration in the salt sensitive hypertension, high salt concentration could exacerbate the renal fibrosis and macrophage infiltration.