变压器噪声暴露对SD大鼠听力及应激状态的影响研究 |
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引用本文: | 易 星,刘兴发,石琳钰,邹 游,戴梦源,陶泽璋,肖伯奎,张建功,陈始明. 变压器噪声暴露对SD大鼠听力及应激状态的影响研究[J]. 现代生物医学进展, 2017, 17(20): 3836-3841 |
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作者姓名: | 易 星 刘兴发 石琳钰 邹 游 戴梦源 陶泽璋 肖伯奎 张建功 陈始明 |
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作者单位: | 武汉大学人民医院耳鼻咽喉头颈外科 湖北 武汉 430060;中国电力科学研究院 湖北 武汉 430070 |
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基金项目: | 国家电网公司总部科技项目(GY71-13-057) |
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摘 要: | 目的:探究短时间内低声级强度低频的变压器噪声暴露对SD大鼠听力及应激状态方面的影响。方法:选取90只SPF级健康无听力障碍的(雌雄各半)SD大鼠作为实验对象,随机分为实验A、B组和对照C组,A、B组分别给予声级上限为65 dB SPL、60 dB SPL(频谱范围:100~800 Hz)的变压器噪声,噪声暴露时程为8周,每日噪声给予时间为22点至次日8点,C组在相同条件下饲养,不给予噪声暴露。噪声暴露结束后,通过DPOAE(畸变耳声发射)、ABR(听性脑干反应)检测、耳蜗铺片及毛细胞计数对SD大鼠听力学状况进行评估;通过血清中促肾上腺皮质激素(ACTH)、血清皮质醇(CORT)对SD大鼠的应激状态进行评估。结果:在变压器噪声暴露的8周内,各组大鼠生长状况良好,体重均呈正常生理性增长,组间无明显差异(P0.05);在变压器噪声暴露8周后,对A、B、C三组大鼠的听力学指标进行两两比较,组间均无明显差异(P0.05),对大鼠血清中促肾上腺皮质激素(ACTH)、血清皮质醇(CORT)的含量进行三组间比较,组间差异均无统计学意义(P0.05)。结论:连续暴露于声压级上限65/60 dB SPL,频谱范围为100~800 Hz的变压器噪声下8周(10小时/天)对SD大鼠听力未产生明显影响,未引发SD大鼠应激状态。
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关 键 词: | 变压器噪声;SD大鼠;听力学;耳蜗铺片与听毛细胞计数;应激 |
收稿时间: | 2017-02-27 |
修稿时间: | 2017-03-19 |
Effects of Transformer Noise on Auditory Function and Stress State in SD Rats |
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Abstract: | ABSTRACT Objective: To determine the effect of short-term transformer noise on auditory function and stress state in SD rats. Methods: 90 healthy SD rats (half male and female) with normal hearing were randomly divided into experimental group A, B and control group C. The experimental groups were exposed to the recorded transformer noise for 8 weeks (10 hours per day, 10:00 pm~8:00 am). Group A was exposed to the pressure level 65 dB SPL at Max (noise spectrum: 100-800 Hz), and group B was exposed to the pressure level 60 dB SPL at Max (noise spectrum: 100-800 Hz), control group C were maintained under the same conditions, without noise stimulation. The indexes of auditory function and stress state of SD rats were determined and compared among the three groups after an 8 weeks'' noise exposure. Results: In the 8 weeks of transformer noise exposure, the rats showed normal physiological growth, and there was no significant difference (P>0.05) among group A, B and C. After noise exposure, indices of the auditory function (distortion product otoacoustic emission, DPOAE; auditory brainstem response, ABR) and the counting and observation of cochlear hair cells of the groups were compared, and no statistically significant difference was observed (P> 0.05). In addition, there were no significant differences in the ACTH and CORT content changes among the serums of three groups (P>0.05). Conclusion: Continuous exposure to transformer noise (sound pressure level 60/65 dB SPL at Max, noise spectrum: 100~800 Hz) for 8 weeks (10 hours/day) had no significant effect on the auditory function of SD rats, and never started the stress state in rats. |
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Keywords: | Transformer noise SD rats Auditory function Cochlear hair cells preparation and count Stress |
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