白藜芦醇对6-羟基多巴所致SN4741细胞损伤的保护作用和机制研究

目的:探讨白藜芦醇对6-羟基多巴引起的细胞损伤的内在保护机制。方法:以SN4741细胞系为实验对象,分为对照组、6-羟基多巴处理组和白藜芦醇预处理、6-羟基多巴处理组组。MTT法测定细胞活性。Western blot检测细胞内DJ-1表达水平。ROS检测反映细胞的氧化应激水平和线粒体损伤情况。线粒体膜电位检测反映细胞线粒体功能。结果:白藜芦醇可以剂量依赖性方式提高6-OHDA诱导的SN4741细胞的存活率。白藜芦醇预处理显著逆转6-OHDA诱导的SN4741细胞DJ-1水平的下降,降低6-OHDA引起的氧化应激水平和线粒体损伤。结论:白藜芦醇预处理能够保护6-羟基多巴所致的SN4741细胞损伤,可能与提高DJ-1的表达,减轻细胞内的氧化应激水平,改善线粒体功能有关。

Resveratrol Protects the Impairment of SN4741 Cells Induced by 6-Hydroxy Dopamine

ABSTRACT Objective: To investigate the mechanism underlying the protection effect of resveratrol against 1. cellular impairment induced by 6-hydroxy dopamine. Methods: SN4741 cells were divided into three groups: the control group, the 6-hydroxy dopamine treated group and the resveratrol pre-treated group. The cellular viability were measured by MTT assay. DJ-1 level were assessed by Western-Blot assay. ROS level, and MMP were detected to reflect cellular oxidative stress level and mitochondrial function. Results: Resveratrol alleviated 6-hydroxy dopamine induced cell death through a dose dependent manner. Pre-treatment of resveratrol significantly relieved the reduction of DJ-1 level induced by 6-hydroxy dopamine, which contributed to a lower oxidative stress level and milder mito- chondrial injury. Conclusion: Pre-treatment of resveratrol protects cellular damage induced by 6-hydroxy dopamine. Moreover, it also alleviates cellular oxidative stress level and improves mitochondrial function. All these effects may due to promoting expression of DJ-1 protein induced by resveratrol.