玻璃体腔联合注射赖氨酸－纤溶酶原和瑞替普酶诱导兔眼玻璃体后脱离的有效性

目的:探讨兔眼玻璃体腔联合注射赖氨酸-纤溶酶原和瑞替普酶诱导玻璃体后脱离(PVD)的有效性。方法:选取30只健康的新西兰白兔,以白兔右眼作为实验眼,左眼作为对照眼。随机分为A、B、C三组(每组10只),三组实验眼分别联合应用1万U瑞替普酶+125μg赖氨酸—纤溶酶原、2万U瑞替普酶+125μg赖氨酸—纤溶酶原、3万U瑞替普酶+125μg赖氨酸—纤溶酶原进行玻璃体腔内注射,对照眼均注射平衡盐溶液。应用视网膜电图、扫描电镜及光镜观察、比较各组诱导PVD的效果。结果:三组实验眼均形成不同程度PVD。A组实验眼注药前与注药后24 h、注药后2周的最大混合反应a波振幅、b波振幅比较均无明显差异(P0.05);B组实验眼注药后24 h的a波振幅、b波振幅均有轻度下降,2周后均恢复正常,注药前后的a波振幅、b波振幅比较均无明显差异(P0.05)。C组实验眼注药后24 h的a波振幅、b波振幅均明显低于注药前和对照眼,注药后2周的b波振幅均明显低于注药前和对照眼(P0.05)。光学组织切片观察显示:A组实验眼及所有对照眼、B组实验眼、C组实验眼的视网膜组织细胞形态正常,结构清晰,但B组神经节内核层、细胞层细胞略有减少,C组神经节内核层及细胞层细胞明显减少。结论:玻璃体腔内联合注射赖氨酸-纤溶酶原和瑞替普酶能有效诱导PVD,1万U瑞替普酶+125μg赖氨酸-纤溶酶原可诱导实现完全性PVD,不会对视功能、视网膜结构造成损害。

The Effectiveness of combined Injection of Intravitreal Lysine Plasminogen and Reteplase-induced Vitreous Body after Detachment in Rabbit

ABSTRACT Objective: To investigate the effectiveness of vitreous body combined with injection of lysine amino acid-plasminogen on the reteplase induce vitreous detachment (PVD). Methods: 30 healthy New Zealand white rabbits were selected, the right eyes were considered as the experimental eye and the left eye were were considered as the control eye. The eyes were random divided into group A, B, C(n=10), three groups of experimental eyes were treated by 1 million U reteplase + 125C mu g lysine, plasmin, 2 million U reteplase + 125C UG g lysine - plasmin, 3 million U reteplase + 125C UG g lysine plasminogen were intravitreal injection, the control eyes were injected with balanced salt solution. The effect of induced posterior detachment of vitreous body were observed and compared. Results: Before injection and at 24h, 2 weeks after injection, the maximum mixing reaction a-wave amplitude and b-wave amplitude showed no significant difference(P>0.05). In group B, at 24 hours after injection, the amplitude of a wave and b wave amplitude were slightly decreased and returned to normal at 2 weeks after injection, the amplitude of a wave and b wave amplitude showed no significant difference before and after the drug injection(P>0.05). At 24 h after injection, a wave amplitude and b wave amplitude of experimental eyes in the group C were significantly lower than those before injection and control eyes, the b wave amplitude at 2 weeks after injection was significantly lower than that before injection and control eyes(P<0.05). Optical biopsy observations showed that the experimental eyes in the group A, B, C and all control group C displayed that the retinal tissue cells were normal, the structure was clear, but group B showed that the the kernel and cell layer of ganglion were slightly reduced, the kernel layer and layer cells of ganglion were significantly decreased in the group C. Conclusion: Intravitreal injection combined with lysine amino acid - plasminogen and reteplase could effectively induce the posterior vitreous detachment, 1 million U reteplase + 125C UG g lysine plasminogen could be induced to achieve complete vitreous detachment, not visual function and structure of the retina damage.