羟苯磺酸钙通过抑制Ⅰ型胶原表达减轻肾间质纤维化

目的:研究羟苯磺酸钙对小鼠肾间质纤维化、Ⅰ型胶原表达的影响。方法:将C57小鼠随机分为假手术组(Sham组,n=4)、肾间质纤维化模型组(UUO组,n=5)及羟苯磺酸钙治疗组(CDT组,n=4);采用单侧输尿管梗阻制备肾间质纤维化模型,CDT组给予羟苯磺酸钙灌胃、Sham组和UUO组给予双蒸水灌胃;采用HE染色、Masson染色、免疫组化、实时定量PCR以及蛋白免疫印迹观察单侧输尿管梗阻术后14 d小鼠术侧肾脏的肾间质纤维化程度和Ⅰ型胶原表达情况。结果:与Sham组比较,UUO组小鼠术后14 d术侧肾脏肾发生显著肾间质纤维化,Ⅰ型胶原表达显著增强(Ⅰ型胶原基因相对表达量:Sham组:1.00000,UUO组:114.92289,P0.0001)。与UUO组比较,CDT组小鼠术后14 d术侧肾间质纤维化程度显著减轻,Ⅰ型胶原表达显著减弱(Ⅰ型胶原基因相对表达量:UUO组:114.92289,CDT组:45.33516,P0.005)。结论:羟苯磺酸钙通过抑制小鼠肾间质Ⅰ型胶原表达从而减轻单侧输尿管结扎小鼠肾间质纤维化。

CalciumDobesilate Alleviate Renal Interstitial Fibrosis by Inhibition of Collagen I

Objective:To investigate the effect of calcium dobesilate on the expression of collagen I in renal interstitial fibrosis inice.Methods:C57 mice were randomly divided into sham operation group (Sham group, n=4), renal interstitial fibrosis model group(UUO group, n=5) and calciumdobesilate treatment group (CDT group, n=4), preparation of renal interstitial fibrosis model by unilateralureteral obstruction (UUO), CDT Group was treated with calcium dobesilate orally, Sham group and UUO group were given doubledistilled water , HE staining, Masson staining, immunohistochemistry, real-time quantitative PCR and western blot was detected after 14days of UUO in mice to observe the renal interstitial fibrosis degree and the expression of collagen I.Results:Compared with the Shamgroup, the renal interstitial fibrosis were significantly increased in the UUO group after operation 14 d, the expression of collagen I wassignificantly enhanced (collagen I gene relative expression: Shamgroup: 1, UUO group: 114.92289, P<0.0001). Compared with the UUOgroup, the degree of renal interstitial fibrosis in CDT group was relieved after operation 14 d, and the expression of collagen I wasdecreased (collagen I gene relative expression: UUO group: 114.92289, CDT group: 45.33516, P<0.005).Conclusion:Calciumdobesilate can reduce renal interstitial fibrosis in mice with UUO by inhibiting the expression of collagen I in mouse renal interstitial.