IL-8, PCT及CRP联合检测在新生儿败血症诊断中的临床意义

目的:探讨血清白细胞介素-8(IL-8)、降钙素原(PCT)及C反应蛋白(CRP)联合检测在新生儿败血症诊断中的临床意义。方法:选择2012年3月-2014年3月我院收治的89例新生儿败血症患儿为观察组,并于同期随机抽取120例在我院出生的健康新生儿为对照组,检测并比较两组新生儿外周血中IL-8、PCT及CRP水平。IL-8采用酶联免疫双抗体夹心法测定,血浆PCT采用免疫层析法检测,CRP采用免疫比浊法检测。结果:观察组新生儿IL-8、PCT及CRP水平均明显高于对照组,差异有统计学意义(P0.05)。三项指标联合检测对新生儿败血症诊断的灵敏度、特异度、阳性预测值、阴性预测值和准确率分别为95.51%、98.33%、97.79%、96.72%和97.13%,均高于三项指标分别进行检测的结果,差异有统计学意义(P0.05)。结论:通过检测外周血中IL-8、PCT及CRP水平可以有效地评估新生儿败血症的病情,三项指标联合检测有助于早期诊断新生儿败血症。

Clinical Significance of IL-8, PCT combined with CRP in Diagnosis of Neonatal Septicemia

ABSTRACT Objective: To explore the clinical significance of serum interleukin-8(IL-8), procalcitonin(PCT)combined with C reac- tion protein(CRP)in diagnosis of neonatal septicemia. Methods: In total of 89 patients with neonatal septicemia as case group were col- lected from 2012 March to 2014 March in our hospital, at the same time, 120 born healthy newborns as control group were collected. De- tection and comparison of IL-8, PCT and CRP in peripheral blood of two groups were launched,in which IL-8 was detected by enzyme linked immune assay double antibody sandwich method, plasma PCT was detected by immunochromatography, CRP was detected by im- mune turbidimetry. Results: The IL-8, PCT, CRP levels in case group were significantly higher than these in control group, the difference was statistically significant(P<0.05). The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of three indicators jointed to detect neonatal septicemia, were higher than these in every indexes, the difference was statistically significant(P<0.05). Conclusion: It can effective evaluate the neonatal septicemia through detecting the IL-8, PCT, CRP levels in peripheral blood, and the three indicators jointed is helpful to early diagnosis neonatal septicemia.