非小细胞肺癌CD166+干细胞的分离和鉴定

目的:分选人非小细胞肺癌中的CD166+细胞,并验证其肿瘤干细胞的特性。方法:收集手术切除的15例非小细胞癌组织标本以及患者的临床资料,并通过流式细胞术对组织中CD166+的表达水平进行检测,对CD166+细胞进行原代培养,对比CD166+细胞和CD166-细胞的成球能力和集落形成能力;并将CD166+细胞和CD166-细胞注射到小鼠体内,比较其体内肿瘤形成能力。结果:在15例临床样本中均检测到CD166+细胞,CD166阳性表达率最高为12.1%,最低为0.3%。CD166的高表达与肿瘤TNM分期、肿瘤转移、以及肿瘤分型密切相关(P均0.05)。CD166+细胞的成球形成能力和集落形成能力显著的高于CD166-细胞,且CD166+细胞在裸鼠体内肿瘤形成能力显著高于CD166-细胞(P均0.05)。结论:非小细胞肺癌CD166+细胞具有肿瘤干细胞的特征,CD166在非小细胞肺癌的发生发展中可能具有重要的作用。

CD166+ Isolation and Identification of Stem Cells in the Non-small Cell Lung Cancer

ABSTRACT Objective: To study the separation of CD166+ cells in human non-small cell lung cancer, and verify the characteristics of cancer stem cells. Methods: To collect surgical removal of the 15 patients with non-small cell carcinoma tissue samples and clinical data, and by flow cytometry to detect CD166+ expression levels in the organization, on the original CD166+ cells in the original generation training, contrast and CD166+/- cells in vitro self-renewal ability; CD166+ cells and CD166- cells injected into mice, differences in the ability of tumor formation in vivo. Results: CD166+ cells were detected in 15 cases of clinical samples, CD166 positive expression rate was as high as 12.1%, the lowest was 0.3%. CD166 high expression was closely related to tumor TNM stage, tumor metastasis and tumor classification (P<0.05). CD166+ cell clone formation ability significantly higher than that of CD166- cells, and CD166+ cells in nude mice tumor formation ability was significantly higher than that of CD166- cells in the body. Conclusion: Non-small cell lung cancer CD166+ cells has the characteristics of cancer stem cells, prompt CD166 in non-small cell lung cancer development may play an important role.