DNA条形码技术在中缅树鼩隆安种群和昆明种群鉴定中的研究

目的:本研究利用线粒体细胞色素C氧化酶亚基I基因(CO1)的一段保守区域作为DNA条形码技术的研究序列,探讨DNA条形码技术对中缅树鼩隆安种群和昆明种群进行分类鉴定的可行性。方法:对22只广西隆安树鼩和21只昆明树鼩样本的CO1基因进行PCR扩增、测序,应用MEGA V5软件对序列进行比对及分析其遗传距离,采用NJ法构建系统发育树。结果:中缅树鼩种群中,隆安种群、昆明种群和海南亚种的种内遗传距离为0.00%-0.79%,种群间遗传距离为9.71%-13.59%,中缅树鼩与普通树鼩的种间遗传距离为20.43%-24.11%,存在条形码间隔。系统发育树显示:隆安种群、昆明种群及海南亚种分别聚为一小支,分支置信度高达100%。结论:本研究结果表明DNA条形码技术有助于树鼩种群和亚种的分类鉴定,经CO1基因的测序分析证实广西隆安树鼩和昆明树鼩分属不同的种群。

DNA Barcode Technology in Identification Study of Longan Population and Kunming Population from Tupaia Belangeri

ABSTRACT Objective: Based on the conservative region of mitochondrial cytochrome C oxidase subunit I gene (CO1) as a DNA barcode technology sequence, the feasibility of DNA barcode technology in identifying Longan and Kunming Population from Tupaia be- langeri was explored in this study. Methods: CO1 gene were amplified by pcr and sequenced in 22 Guangxi Longan and 21 Kunming tree shrew samples. Sequences were aligned and genetic distance was measured by MEGA V5. The phylogenetic trees were constructed by NJ method. Results: The intraspecific genetic distance of Longan population, Kunming population and T.belangeri modesta from Tupaia belangeri was 0.00%-0.79%, the interspecific genetic distance between Tupaia belangeri subspecies was 9.71%-13.59%, and the interspe- cific genetic distance between Tupaia belangeri and Tupaia glis was 20.43%-24.11%. There is barcode gap. Phylogenetic tree showed that Longan population, Kunming population and T.belangeri modesta from Tupaia belangeri were clustered into three small branches, branches of confidence as high as 100%. Conclusion: The results showed that DNA barcode technology is helpful to the classification and identification of tree shrew species and subspecies. Through the CO1 gene sequencing analysis, the study confirmes that Longan pop- ulation and Kunming population from Tupaia belangeri are belong to different species.