乳腺癌体外骨转移瘤3D模型的建立

目的:构建乳腺癌体外骨转移瘤3D模型。方法:新生CD-1小鼠的颅骨单独孵育为正常组,新生CD-1小鼠的颅骨与MDA-MB-231细胞低氧共孵育四天为模型组。通过扫描电镜(SEM)鉴定骨转移瘤模型,中性红染色法鉴定骨组织中的破骨细胞,硝酸银复染法观察骨的溶解,结晶紫染色法观察骨转移瘤模型中肿瘤细胞的生长。结果:正常组骨表面光滑完整;模型组骨组织表面黏附大量肿瘤细胞,破骨细胞活性增强,产生严重的溶骨,表面出现骨陷窝,骨纤维发生断裂。结论:成功建立了乳腺癌骨转移瘤体外3D模型,该模型能够模拟体内骨转移瘤微环境。

Construction of Ex-vivo 3D Breast Cancer-bone Metastasis Model

ABSTRACT Objective: This study aimed to construct an ex-vivo 3D breast cancer bone metastasis model. Caldaria which incubated with MDA-MB-231 cells for 4 days in a hypoxia systems as model group. Methods: Calvaria from neonatal CD-1 mice as normal group, which incubated with MDA-MB-231 cells for 4 days in a hypoxia systems model group. The bone morphology and bone resorption of advanced breast cancer with bone metastasis were detected by scanning electron microscope (SEM) to authenticate the success of model, NR staining to distinguish the osteoclast, silver nitrate counter staining to observe the osteolysis and crystal violet staining to observe the growth of tumor cells in bone metastases site. Results: The surface of bone tissue was smooth in normal group. After calvaria incubated with MDA-MB-231 cells for 4 days, the bone fiber was fractured, bone lacuna was developed, the activity of osteoclast was increased and the osteolysis was severe. Conclusion: The ex-vivo 3D breast cancer-bone metastasis model successfully simulated bone metastasis in-vivo micro-environment.